• Facebook
  • YouTube
  • Twitter
  • Flickr
Home | Classifieds | Useful Files | Links | 01948 666295 *

Epididymal Freezing

Post Castration Epididymal Sperm Extraction
‘It’s not too late’



When should this technique be used?

Post castration epididymal semen extraction is a pioneering method of semen collection that permits semen extraction where other options don’t allow. At Stallion AI Services, we have carried out over 100 extractions, banking and saving the genetics and breeding potential of stallions that otherwise would have been lost.

The technique can be used following routine castration, upon death of the animal or where the testes have suffered a severe trauma or testicular torsion. The procedure provides a last chance method of salvaging semen when there is no other option available.


The stallion has one or both testes removed which are then chilled and couriered to the semen collection centre. The testes are dissected and the sperm cells harvested from the epididymis using a combination of techniques including aspiration, flushing and flotation.

How many doses will be harvested?

The number of doses collected varies from stallion to stallion depending on testicle size and density of semen collected. On average, the final number of doses varies from 10-40 based on harvesting from both testes.

Does the stallion need health testing?

All stallions must be tested for EVA, EIA and CEM. This should be done in advance where possible but can be taken at the point of castration.

Does it work?

Although there is not an abundance of data on the fertility of semen harvested from the epididymis, using a variety of specialised processing techniques, we are now seeing post thaw results of up to 50% progressive motility and positive pregnancy results have been achieved.

Picture Courtesy of Gallery South

Stallion AI Services Recommendations:

Whilst epididymal semen extraction is a highly beneficial method of extracting semen in non-planned emergency situations, it is not recommended as an alternative to ejaculated semen freezing. Due to the factors that can affect the semen quality, it should be viewed as a last chance method of semen harvesting. If situations permit for collection and freezing of ejaculated semen prior to castration of the stallion, this should always be recommended as the primary course of action.

Important Information for Veterinarians

The success of this procedure is very much dependent on the speed at which the testes are transported to our centre from the time the animal was castrated or the time of death.  It is important that the testes are washed and packaged correctly.  It is advised that all blood is washed from the testes prior to transportation and that when possible the vas deferens is clamped.  The testes should be kept chilled, but must not come into direct contact with ice or ice packs.

For more detailed information on this procedure please contact the lab on 01948 666295 or email lab@stallionai.com